2021-01-15

A method for collection of subgingival plaque and calculus. Oshrain HI, Salind A, Mandel ID. PMID: 5247121 [Indexed for MEDLINE] MeSH terms. Dental Calculus/analysis* Dental Plaque/analysis* Humans; Methods; Models, Structural; Specimen Handling

However, it is essentially impossible to collect sufficient volumes of gingival fluid for coating the hydroxyapatite disks. For this reason, we elected to use unstimulated saliva. Background: The collection of subgingival plaque samples with paper points is time‐consuming and accident‐sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results A total of 1,170 samples of supra and subgingival plaque were collected from the mesial aspect of every tooth (up to 28 supra and 28 subgingival samples) from each subject and evaluated for the plastic tubes during the final 5 min. Supragingival plaque samples were collected and pooled by sterile curettes from all teeth surfaces (6.5 ± 0.9 teeth), on the side of the upper half-jaw that contained the most teeth.

Subgingival plaque collection

1. Sample Collection. Buccal and subgingival plaque samples were collected 1 week after the full-mouth periodontal examination. All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling.

Subgingival plaque sample collection. For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently.

The site was then cleaned and isolated using cotton roles and air dried gently. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria.

Collection of subgingival plaques Subgingival plaquesampleswerecollectedby asterile hand scalerandtransferred ineither one ml of sterile reduced transfer ﬂuid (RTF) consisting of 0.01% dithiothreitol in PBS, pH 7.4 or one ml of distilled water. The samples were immediately placed at 4 C and analyzed within 4 h.

MID (Motivation, Instruktion och MID, plaque registrering, fickstatus, mobilitet, furkar, BVS. 2.

Periodontist and laboratory personnel were unaware of case or control status. Four samples (subgingival and supragingival plaques, saliva, and tongue coating) per each subject were collected from 14 patients with a broad range of severity of periodontitis before periodontal therapy. The bacterial composition was analyzed by 16S rRNA gene amplicon sequencing using Ion PGM. Clinical assessments by supragingival plaque (PL+), gingival index (GI), bleeding on probing (BOP), probing depth (PD), clinical attachment level (CAL) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. A total of 1,170 samples of supra and subgingival plaque were collected from the mesial aspect of every tooth (up to 28 supra and 28 subgingival samples) from each subject and evaluated for the The sulcular circumferential sweep method described for detecting subgingival plaque is done by placing the probe gently into the pocket/sulcus until it reaches the epithelial attachment, and then drawing the probe horizontally with the tip remaining at the base of the pocket/sulcus. Subgingival plaque sample collection: Removal of supragingival plaque with sterile cotton pellets and isolation of teeth with cotton rolls was done.
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De novo subgingival plaque formation The introduction of oral implants especially a two stage type, provides a new experimental setup.

A Method for Collection of Subgingival Plaque and Calculus Herbert I. Oshrain D.D.S. Section of Periodontology and Division of Stomatology, School of Dental and Oral Surgery, Columbia University, New York. The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface.
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GCF was collected by using an intracrevicular washing technique PCR analyses of subgingival plaque samples from subjects with and without bad breath.

The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria.

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2015-11-27 · To explore the ultrastructure of subgingival dental plaque using high-resolution field emission scanning electron microscopy (FE-SEM) and to investigate whether extracellular DNA (eDNA) could be

av G Dahlén — av provtagningspinnar och kommersiella provtagningsset dental plaque of hospitalized patients with chronic lung diseases. Clin subgingival plaque.